Transplantation with adipose tissue derived stem cells increased follicle survival in frozen–thawed human ovarian tissue.
A recent paper published in 'Human Reproduction' by Diego Manavella et al looked at a two-step transplantation process using adipose tissue derived stem cells in which they found increased follicle survival by enhancing vascularization in xenografted frozen–thawed human ovarian tissue.
The team from the Gynaecology Research Unit, Institut de Recherche Expérimentale et Clinique, UCL Louvain, who use Planer controlled rate freezers, found that higher rates of oxygenation and vascularization of ovarian tissue, as well as increased follicle survival rates, were detected in the early post-grafting period. The results suggested that the proposed transplantation procedure with ASCs is a promising step towards potentially solving the problem of massive follicle loss after ovarian tissue grafting.
For further information
Article: Human Reproduction, Volume 33, Issue 6, 1 June 2018, Pages 1107–1116,
We are delighted to introduce Phil Crow who joined the Planer sales team on 1st June as the International Sales Manager for Asia and the Middle East.
Phil brings to Planer a vast array of experience and knowledge of international distributor management in the healthcare industry, most recently having worked as the National Sales Manager at Apex Medical. He describes himself as a highly motivated and driven sales professional who enjoys the challenge of providing a high level of customer satisfaction.
Phil and his wife Deborah like to spend their down time appreciating good food, live entertainment and the movies, anything from the classics to the latest blockbuster; but most of all, providing he's allowed, Phil enjoys his one escape from the rat race on the golf course. A big fan of fictional crime novels, Phil is currently reading his way through author Simon Kernicks library of fast paced bestsellers.
Planer will once again be exhibiting at the European Society of Human Reproduction and Embryology (ESHRE) conference in 2018. This year the conference is being held at the Centre de Convencions Internacional de Barcelona (CCIB) in Barcelona, Spain from the 1st to the 4th July.
We would welcome any of our distributors and their customers to come and see us at this year’s conference where we will have the CT37stax™ multi chamber incubator and DATAssure™alarm and monitoring system on display alongside a range of cryogenic storage vessels suitable for the ART market.
We look forward to seeing many of you at this important event in the ART calendar. Please do let us know if you are planning on attending - we will be on stand 386.
This year, the biennial meeting of ALPHA, the international society aimed at advancing the science of clinical embryology around the world, will be taking place in the beautiful city of Reykjavik, Iceland between 17th and 20th May. Planer will be attending the meeting – if you are going, please do come and find out more about our latest range of products.
CT37stax™ multi chamber incubator
Our CT37stax™ incubator complements the existing range of well-established Planer benchtop incubators. It is a high capacity incubator, which has been designed using the best features of the existing technology and incorporates these with a mix of innovative new features to provide a state of art new multi chamber benchtop incubator fit for the demands of the modern laboratory.
We are delighted to announce that the CT37stax™ has just gained medical device accreditation under the Medical Devices Directive 93/42/ECC - which now allows this product to be sold for human applications e.g. ART applications in all countries covered by these regulations.
DATAssure™ wireless monitoring system
The Planer DATAssure™ wireless monitoring system is used across a broad range of market sectors meeting the most stringent standards to assist customers to comply with HACCP, BRC, FDA and MRHA legislative requirements. The system is standalone and connects directly to your business IT network with no need for any dedicated PC, server or specialist installed software. Data from the base station can be viewed direct on the colour touch screen or via a standard web browser.
Our ShipsLog3™ is a temperature data logger that provides an accurate and downloadable temperature history of your vapour shipper throughout its transit. It measures and stores more than 32,000 temperature readings from the thermocouple fitted to the inside of the shipper lid. This will monitor and record the temperature of the shipper at the warmest part ensuring the samples in the core of the shipper are stored at the correct temperature and providing early alerts of potential warming issues.
If you are unable to attend ALPHA, but would like to find out more about our range of products suitable for the clinical embryology field, please do get in touch. We would be delighted to hear from you.
A study on these freezing responses, relating to aggregates versus single cells, has just been published in Tissue Engineering (on-line February 2018) by Rui Li, Guanglin Yu (pictured here) and others working with Prof Allison Hubel's team at the Department of Mechanical Engineering University of Minnesota. Human induced pluripotent stem cells (hiPSCs) are multicellular aggregates attracting much interest in tissue engineering, disease modelling and personalised medicine. They can be frozen either as aggregates or single cells depending upon the application. For both clinical and scientific purposes, effective cryopreservation of hiPSCs is required for transportation, storage of frozen hiPSCs and other downstream uses. However, cryopreserved hiPSCs are vulnerable to the loss of viability, function or pluripotency.
It is known that inadequate preservation methods of hiPSCs have impeded efficient re-establishment of cell culture after their freeze-thaw. In the study the roles of cooling rate, seeding temperature and the difference between cell aggregates and single cells in controlled rate freezing were examined using, inter alia, Raman spectroscopy, as a tool for understanding cell responses to the freezing environment. The Raman spectroscopy was used to observe both hiPSC single cells and aggregates frozen at three cooling rates and two seeding temperatures; it suggested higher sensitivity of aggregates to supercooling than previously thought. The work will deepen understanding of behaviours of single cells and aggregates frozen at various conditions and help promote the development of improved cryopreservation protocols for human induced pluripotent stem cells.
For single cells, slow cooling rates allowed significantly better preservation of membrane integrity than higher cooling rate (10˚C/min) regardless of the seeding temperature. For aggregates, however, slow cooling rates (1, 3˚C/min) combined with high seeding temperature (4˚C) had little effect on the membrane integrity but resulted in significantly better cell attachment than higher cooling rate (10˚C/min) or low seeding temperature (8˚C). The authors say there are advantages of using a seeding temperature of 4˚C compared to 8˚C suggesting that the range of seeding temperatures of 7˚C to 12˚C quoted in much literature may be sub-optimal, and that seeding temperature should be considered as a critical parameter when designing cryopreservation protocol for hiPSCs. Guanglin Yu, see photo above, carried out a lot of the experimentation. He said "...we used manual seeding for nucleation of the sample. We sprayed the sample with a narrow stream of liquid nitrogen. We saw different cell responses of HiPSC with seeding temperature of -4˚C and -8˚C using Raman microscopy. This made it clear that we wanted to control the temperature at which ice formed in the extracellular space for controlled rate freezing experiments."
The paper indicates that hiPSCs respond to freezing in very complex fashion, and successful establishment of post thaw culture depends on various critical factors. Further studies will need to not only continue exploring additional factors to optimise the freezing protocol for hiPSCs but investigate the biological pathways connecting the factors and the observed cryopreservation outcomes to provide targets for future development of cryoprotectants.
For further information
Tissue Engineering: https://www.liebertpub.com/doi/abs/10.1089/ten.TEC.2017.0531
Planer Controlled Rate Freezer: https://planer.com/products/cryo-freezers/medium-crf.html
On the 34th anniversary of the birth of the first baby from a frozen embryo, the BBC World Service broadcast an interview with Alan Trounson the pioneer doctor who undertook the procedure.
Zoe Leyland was born in Melbourne, Australia on 28th March 1984, helped on her way by Drs Trounson and Wood who made medical history. The decision to try 'test tube' fertilisation and embryo freezing was taken by Zoe's parents – her mother a 33 year old New Zealander and father a 38 year old British born Australian resident. Her mother had hormonal stimulation and produced eleven eggs which were frozen using a then new type of controlled rate freezer made by Planer plc. One of those frozen embryos became Zoe - who weighed in at about 5 lbs or 2.5 kilos.
The world's first 'fresh’ test tube baby was Louise Brown born in England in 1978, but Zoe came from an embryo frozen for a time before being thawed and implanted. To allow cells to survive liquid nitrogen temperatures (-196°C) the embryos had to be treated with cryo-protectant, then slowly frozen down in the Planer freezer with extreme precision using different temperature ramps, before being stored in liquid nitrogen. This controlled rate freezing procedure is now used in cell laboratories worldwide. The controlled rate freezing technique, originally suggested some fifty years ago by British Scientist Professor David Pegg, enabled Planer plc to pioneer the equipment and today many thousands of units are in constant use all over the world in stem cell labs, IVF, hospitals and research institutions. This controlled rate freezing is often needed before storing certain types of cells in liquid nitrogen – in areas such as cord blood banking, bone marrow transplants, botanical matter, semen, oocytes, botanical seeds, skin, ovarian tissue, heart valves and blood vessels.
The AVA Clinic in Latvia was opened in 2005. The experienced team get high infertility treatment results, and as they adhere strictly to ISO 9001:2008 , the same rigour must apply to safety in their cryo store - which is why they chose our O2NE+ low oxygen alarm system.
In the unlikely event that there were to be a leak in the cryo store causing a build-up of nitrogen gas, which is colourless and odourless, the depletion of oxygen levels might pose a serious danger. The O2NE+ system provides two audio visual alarms which are usually pre-set at 19.5% & 18% to warn personnel of a potential hazard should the oxygen levels deplete. The main senor unit is wall mounted and repeater units can be sited at other entrances. The system has a long life O2 sensor, allowing simple calibration with low maintenance.
Andris Grunskis, pictured here, embryologist at AVA, is also the Quality Systems Manager who specified the O2NE. He graduated from the University of Latvia with a Master’s degree in Biology and regularly participates in international conferences and hands-on workshops and is a member of European Society of Human Reproduction and Embryology and the Nordic Fertility Society. " ... your engineers were great!" he commented after installation.
The 8th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2018) will be held at Taipei International Convention Center, Taipei, Taiwan from 12 - 15 April 2018. Over the three days, the conference will consider the latest developments and their impact on the future of fertility preservation, embryology, andrology, clinical trials, PCOS, PGS, IVM, and related fields.
Planer will be exhibiting at Aspire 2018. If you are planning to visit the conference, don’t forget to come and see us on stand C53. We will be displaying the full range of Planer IVF products, including the CT37stax™ multi chamber incubator, the DATAssure™ wireless alarm and monitoring system as well as Shipslog3™ and PetriSense®.