The Tri-Gas Mixer is a digital CO2-O2 controller that mixes CO2, air and N2 to the desired concentration ranging between 0-10 % for both CO2 and O2, and at controlled pressure in the range of 0-2 barg (0-30 psig).
Delivery pressure is easily regulated by adjusting the knob of the embedded pressure gauge. The device is equipped with a mixing tank to ensure the highest composition stability even when the required output flow is variable.
Compatible with all benchtop incubators
The Tri-Gas Mixer is compatible with any benchtop incubator available on the market. Models are available with maximum output flow rate of 1.5 L/min and 15 L/min. The actual flow rate delivered automatically adjusts to match the requirements of the connected equipment.
At the end of December 2019, following the acquisition of Planer by Hamilton Thorne Limited earlier in the year, Geoffrey Planer retired from his role as Chairman.
During his 45 years at the helm, Geoffrey led Planer to become an industry market leader, regarded by many as the “go-to” company for scientific and technical expertise when developing a new product or technique. Over the years, Planer equipment has been used in many notable scientific firsts — in 1984, the first baby born from a frozen embryo used a Planer controlled rate freezer. More recently, our freezers have been used in the significant advances made in ovarian tissue freezing.
Today, thanks to Geoffrey’s leadership and expertise over the past four decades, Planer is now a global brand, excelling in the design, manufacture, supply and support of medical products in the assisted reproduction, stem cell and cryopreservation markets.
I am sure many of you will have come into contact with Geoffrey at some point and will remember his friendly, warm manner and smiling face whenever you met him, either in Sunbury or at one our meetings around the world.
Geoffrey will be greatly missed by everybody at Planer: many of the team worked with him for over 20 years. At our recent Christmas lunch, we were delighted to have the opportunity to make a presentation as a small token of our appreciation and to thank him personally for his support and encouragement over the past years.
I am sure you will join us all in wishing Geoffrey well in his retirement. I know his wife Jan is looking forward to having him at home for dinner before 8:30pm every day.
Geoffrey, Good Luck — and a huge thank you from all your colleagues and friends around the world.
Currently 1.2 million patients are receiving treatment from regenerative medicine products produced by over 150 companies with a capital value of around $4.7 billion. Most molecules, cells and tissues are collected at a given time and location for use at a later time. Therefore, our ability to stabilise biological properties (e.g. viability, biomarkers) during transportation and long-term storage is a critical technology.
"Preservation of cellular therapies" course
The University of Minnesota Biopreservation Core Resource (BioCoR) will be running another of their successful "Preservation of cellular therapies" courses on 18th-19th May 2020. The programme will cover the fundamentals of preservation, protocol development, design of a storage facility, regulatory issues associated with preservation of cell therapies, clinical issues and more. Allison Hubel, (pictured here) PhD, Professor of Mechanical Engineering and Director of the Biopreservation Core Resource (BioCoR, www.biocor.umn.edu), will be one of the programme lecturers.
Who should attend?
The course material is designed for those who have little experience with preservation, as well as those proficient in preservation who is interested in improving their practices. The preservation of cells has applications in the fields of recombinant cell biotechnology, cell banking, cell therapy, regenerative medicine and cell-based assays.
We are pleased to announce that Planer is now the UK distributor for the Hamilton Thorne Clinical and Research Laser Range, including the LYKOS laser. If you are going to Fertility 2020, do drop by Stand One, to find out more.
The LYKOS laser, with its Dynamic Targeting System (DTS), represents the next generation of clinical lasers for assisted reproductive technology:-
Designed for ART
The LYKOS laser, with its precise targeting features, automated calibration and built-in quality control reporting, provides computer-controlled accuracy and ease-of-use.
The automated initialisation process calibrates the system and can be run any time from the control panel. This initialisation process maps the position of the RED-i target in relation to the entire field of view to optimise accuracy.
Quality control is a vital part of laboratory procedure. The DTS positioning can be easily verified prior to use and the results of the verification saved. With the built-in quality control reporting, you can view the results of the daily verification in both numerical and graphical form. In addition, a Trend Chart lets you see the results over a selected period of time.
The RED-i target locator is visible both on the screen and through the microscope eyepieces. As well as being used to identify and calibrate the laser position under DTS mode, RED-i allows positioning of the cell under the laser beam without looking at the monitor. The target spot always remains in focus and has an adjustable brightness level.
Find out more
Ovarian tissue cryopreservation and transplantation techniques are rapidly becoming an accepted path for fertility preservation of prepubescence girls and women that require cancer treatment that would otherwise leave them unable to have families of their own.
To date, more than 130 children worldwide have been born using this new technique with by far the majority of these successful outcomes resulting from tissue frozen using a slow freezing process in a controlled rate freezer.
In our recently published “Ovarian Tissue Cryopreservation Scientific Round-Up” two different procedures are described - the first outlines the work from a combined team from Singapore’s Sincere IVF and Gleneagles Hospital whilst the second gives an overview of the approach used by Belgium’s Université Catholique de Louvain.
Ovarian tissue freezing has been used clinically for fertility preservation in children, adolescents and adults with cancer since 2004, following the world’s first live birth using the cryopreservation and transplantation of ovarian tissue.
Whilst the procedure has been accepted in an increasing number of countries, it is still a relatively new procedure, within the area of assisted reproduction technologies.
Why use ovarian tissue cryopreservation and transplantation?
Ovarian tissue cryopreservation represents a new technique to preserve fertility in women where these normal fertility preservation techniques are not an appropriate option.
Currently, embryo and oocyte cryopreservation are the only fertility preservation techniques considered by the American Society for Reproductive Medicine not to be classified as experimental. Unfortunately, there are several reasons why one of these approaches may not be appropriate for some patients:
For these patients, the possibility of cryopreservation of ovarian tissue (cortex) has become an urgent and highly-demanded technology.
Ovarian tissue cryopreservation and transplantation does not require ovarian stimulation and offers a promising option for women at high risk of premature ovarian failure and sterility. Furthermore, transplanting ovarian tissue not only restores fertility but also restores endocrine function.
Slow freezing versus vitrification
Ovarian tissue freezing and transplantation, using a controlled rate freezer, has a number of advantages over vitrification. The automated process of a programmable freezer allows repeatability, with an auditable trail, hence reducing the risk of human error. Slow freezing also allows larger grafts of ovarian tissue, which could be important in improving success rates.
Download our Ovarian Tissue Cryopreservation Scientific Round-Up
To find out more about the combined team from Singapore’s Sincere IVF and Gleneagles Hospital and the Université Catholique de Louvain’s approaches, download our Ovarian Tissue Cryopreservation and Transplantation Scientific Round-Up.
For further information
Ovarian tissue freezing
Successful oocyte retrieval and fertilisation after transplantation of cryopreserved ovarian tissue
New ovarian tissue freezing programme
Cancer cells purged from human ovarian tissue fragments by pharmacological inhibition of YAP/TAZ oncoproteins
First birth in Italy from cryopreserved ovarian tissue transplanted to a cancer patient
Increased follicle survival in frozen–thawed human ovarian tissue
First British woman gives birth after an ovarian tissue transplant
Unique birth from ovarian tissue frozen in 2001
We are delighted to announce that Planer is now the UK distributor for the Gynemed range of media products. If you are going to Fertility 2020, do drop by Stand One, to find out more.
The Fertility 2020 Conference, organised by the Association of Clinical Embryologists, British Fertility Society and the Society for Reproduction and Fertility, will take place in Edinburgh’s International Convention Centre from the 9th to the 11th January. The theme this year is “Reproduction in changing world” and will feature speakers from around the world, talking on fertility and reproductive biology.
Planer Ltd is now part of the Hamilton Thorne Group and we are now the UK distributor for the Hamilton Thorne Clinical and Research Laser Range, along with the Gynemed range of consumable and media products.
While at the Conference, come and talk to the Planer team on Stand One to find out more about Gynemed Media, designed for:-
At Planer, we are frequently asked the question “How do we optimise our freezing profile for our controlled rate freezer to get the best results?”. We thought that a summary of a recent presentation by Allison Hubel, might provide some useful guidance. Allison, an expert in Cryobiology from the University of Minnesota, USA, gave an informative talk to the Stem Cell users Group and the Society of Low Temperature Biology meeting in Seville where she explained the key factors to consider when trying to optimise a freezing profile. Here is an outline of her presentation on "Optimising Freezing Profiles"
Cryopreservation of a cell therapy
Cells therapies are becoming a standard of care for the treatment of disease and injury. Unlike other standard therapies (i.e. drugs and medical devices), cell therapies have a complex supply chain that requires viable, functional cells all along it. It is noteworthy that what happens along the supply and processing chain determines the quality of the product at the end. Cryopreservation of a cell therapy is a common method of stabilisation along this complex supply chain.
Cryopreservation typically requires 6 steps:
(1) Pre-freeze processing
(2) Formulation and introduction of a cryopreservation solution
(6) Post thaw characterisation.
Optimizing the freezing process
It has been known for almost 50 years that the rate of cooling has a strong influence on the post thaw recovery of cells. Controlled rate freezing is often used to control the temperature as a function of time for cells being cooled; therefore improving both the recovery and consistency of outcome.
A controlled cooling rate protocol has 5 steps:
(1) Initial equilibration
(3) Seeding of the sample
(4) Secondary cooling
(5) Cooling to the final temperature.
The initial equilibration stage of the freezing protocol enables samples placed in the controlled rate freezer to equilibrate with the freezer. Optimising this step, in particular, helps to improve reproducibility of the freezing process. The cooling rate for the sample is the cooling rate used for both primary and secondary cooling of the sample. One degree C/min is a cooling rate commonly used for several cell types.
Controlling the temperature at which ice forms in the extracellular solution
The temperature at which ice forms in the extracellular solution also plays an important role in the post thaw survival of cells. There are several methods of controlling the temperature at which ice forms in the extracellular solution: manual and automatic seeding. Manual seeding requires the use of liquid nitrogen or a chilled instrument to induce nucleation and this approach is commonly used with cell types that require precise control of the nucleation temperature. Automatic seeding uses a dip in the sample temperature to induce nucleation. Ultimately, the sample is cooled to a final temperature at which time it is transferred to a low temperature storage unit.
Debugging your freezing protocol
It is important to note that debugging your freezing protocol is straightforward; you can at any time stop the process, thaw the sample and determine viability. This approach allows us to determine if a particular segment of the controlled rate freezing protocol results in cell losses and a basis from which to change and optimise the protocol. Developing strategies for optimising and debugging freezing protocols will be critical in the development and implementation of high efficiency cryopreservation protocols.
Allison's presentation at the 2019 SLTB meeting was extremely well received and generated some thought-provoking discussions during the meeting. For more information, her book "Preservation of cells. A practical manual" provides an extremely useful guide to explore the subject in greater detail.
For further information
More about Allison Hubel
"Preservation of cells. A practical manual" by Allison Hubel
BioCoR, University of Minnesota, and the science, technology and practice of bio preservation
Society of Low Temperature Biology
Liver disease kills more than 12,000 people in the UK each year. As deterioration from liver disease is extremely rapid, any cell therapies to treat the disease must be readily available. A bioartificial liver machine (BAL) can provide the liver function and allows patient livers to regenerate.
The team at University College London have developed a BAL based on alginate encapsulated liver spheroids (AELS) and cryopreservation in >1 litre cryobags. The cryopreservation method enables long term storage and fast delivery of BAL to patients.
At the recent Society of Low Temperature Biology meeting in Seville, the UCL team presented a poster “Does DMSO toxicity in cryopreservation of liver cell derived organoid culture?”. Their approach is intended to help optimise a process for large scale biomass cryopreservation, suitable for a clinical bioartificial liver machine.
The team concluded that AELS can be exposed to 12% dimethyl sulfoxide (DMSO) for up to 10 minutes in temperatures of up to 37 °C without compromise to their viability of viable cell number. However, high concentration DMSO (40%) is toxic for the cells and significantly affects cell viability and cell number immediately after treatment after only a short exposure.
For further information
To download the poster, please click here
Progress on a bio-artificial liver
Cryopreservation for the UCL bioartificial liver
The Liver Group Charity
Society for Low Temperature Biology (SLTB)